Raw-blue cells

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August undefined, 2024

WebSep 5, 2024 · RAW-Blue cells and HEK 293 h-TLR transfected cells Murine RAW-Blue, HEK-BLUE-hTLR2 and HEK-BLUE-hTLR4 cells carrying a SEAP reporter construct were purchased from InvivoGen (San Diego, CA, USA). WebJan 16, 2024 · Then you can follow this protocol: 1- Plate 1–2 * 10^4 adherent cells or 1–2 * 10^4 to 1 * 10^5 suspension cells in 100μl per well of a 96-well plate. Incubate overnight. 2- Allow the TR to ...

What are the validation parameters for RAW BLUE macrophages …

WebNational Center for Biotechnology Information WebSize. 3-7 x 10e6 cells. Contact us. Ask RAW ™ cells selection audit. Properties. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Conjugation. Blue Substrate. east coast military bases map

RAW-Blue ISG Cells - InvivoGen

WebJun 30, 2011 · During the course of blocking CD36 oxLDL binding function using anti CD36 antibodies, we have identified a novel domain of CD36 that triggers inflammatory response-independent of oxLDL binding. OxLDL bound to the mouse reporter cell line RAW-Blue induced TNF-α and RANTES mRNA and protein expression. Pretreatment of RAW-Blue … WebThe in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor ... WebSep 6, 2024 · RAW-Blue and THP-1-Blue cells were plated at 1x10 6 cells/ml in flat bottom 96-well plates in a 100 µL volume. Excipients and TLR ligands were added for a final volume of 200 μL. After 24 h of stimulation, supernatants were collected and NF-κB activation was measured using the QUANTI-Blue detection medium according to manufacturer … cube shop uslar

Inflammatory biomarkers on an LPS-induced RAW 264.7 cell …

RAW Blue cells - Gentaur.com

WebRAW-Blue™ ISG cells allow the detection of bioactive murine type I IFNs or PAMPs by monitoring the activation of the interferon regulatory factor (IRF) pathway. They are derived from the RAW 264.7 macrophages. RAW-Blue™ ISG cells stably express a secreted embryonic alkaline phosphatase (SEAP) gene under the control of the interferon-stimulated WebIn the present study, we used murine macrophage RAW-Blue cells, which are chromosomally integrated with secreted embryonic alkaline phosphatase (SEAP) inducible by NF-κB. We confirmed the expression of TLR4 mRNA and protein in RAW-Blue cells by RT-PCR and Western blot, respectively. east coast minibus hireWebOct 1, 2024 · Results to characterize the IFN response time of the RAW-Blue ISG ™ (RAW-Blue) and B16-BlueIFN-α/β™ (B16-Blue) cells to IFNα and IFNβ and SEAP catalyzed Quantiblue™ (QB) development time are shown in Fig. 1, Fig. 2, Fig. 3.In Fig. 1 a range of IFN concentrations of 1 to 10,000 Unit/ml (U/ml) were added to each cell type and incubated … cubeside stats mod

"WebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. " - Raw-blue cells

Raw-blue cells

WebJan 26, 2024 · RAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern ... WebLipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. The reagent allows for transfection to be performed both in presence or absence of serum. The easy protocol, exceptional plasmid DNA delivery, and the ability to ...

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WebJan 21, 2024 · Raw-blue™ cells were cultured at 37 °C using manufacturer recommendations (InvivoGen) in a 24-well microplate until 70–80% confluence (1 × 10 5 cells/well) was reached. This cell line expressed the secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by the transcription … WebNational Center for Biotechnology Information

WebRAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This cell line is a commonly used model of mouse macrophages for the study of cellular responses to microbes and their products. InvivoGen has developed RAW-Blue ™ cells and RAW-Lucia ™ cells, which express a secreted inducible ...

WebRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the activation of the endogenous MIP-2 promoter. MIP‑2, also known as CXCL2, is the murine homolog of human IL-8, a chemokine produced in an NF-κB-dependent manner [3]. WebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ...

WebJun 29, 2024 · RAW-Blue macrophages were purchased from InvivoGen (San Diego, CA). All cells were propagated in RPMI 1640 supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C in a 5% CO 2 incubator [16, 17]. Cell viability assay. Cell death was quantified by the MTT reduction assay. Cell viability was quantified by the Alamar …

WebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C). cubeside webinterfaceWebSep 30, 2024 · Supernatant from the treated cells was collected and SEAP production was detected over a period of 45 minutes at 655 nm using the QUANTI-Blue ™ detection medium. Detection of protein expression by immunoblotting and densitometry. Cells from the treated well-plate were lysed and saved in the -80 ° C freezer for east coast mining llcWebRAW-Blue™ cells were derived from the murine RAW 264.7 macrophages with chromosomal integration of a SEAP reporter construct inducible by NF-κB. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including all toll-like receptors (TLRs) except for TLR5, but with TLR4 and TLR8 expressed at the highest levels. east coast ministers conferenceWebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture. east coast miningWebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … east coast mini donuts food truck menuWebDec 20, 2024 · The measured mTLR9 activity value of all the synthesized 24-mer ODNs in the dataset. The ODNs were divided into two groups of high (shown in purple) and low (shown in green) activity using a cutoff score of 0.4, based on the optimal density (OD) results from the Raw-blue reporter cell assay cubeside shopWebApr 6, 2024 · RAW 264.7 was inoculated onto a 96-well plate at a density of 2x10^4 cells/well, incubated for 24 h under the above conditions. Then, I stimulated RAW with LPS (1 ug/ml) after 1 hour of drug ... east coast mini classics