WebUsing 3C-based experiments in B cells where CTCF protein levels were downregulated by shRNA-mediated CTCF depletion or Mb1-Cre-mediated conditional deletion of the Ctcf gene in vivo, we and others have shown that in the absence of CTCF IgH locus contraction is only modestly affected (Degner et al., 2009) and Igκ long-range chromatin ... WebApr 10, 2024 · In parallel, an unbiased in vivo shRNA screen to identify regulatory transcription factors of memory CD8 + T cell differentiation further confirmed the role of CTCF in CD8 + T cell differentiation (Figures S3 B and S3C; Tables S4 and S5). shRNA constructs targeting CTCF were enriched in the T EM and T CM populations …
Noncoding transcription within the Igh distal VH region at PAIR ... - PNAS
WebNov 5, 2012 · Mutation of CTCF-binding sites in plasmid-borne promoters reduced transcriptional activity in an orientation-dependent manner. Depletion of CTCF by … WebAug 23, 2013 · Lentiviral particles containing shRNA of CTCF tagged with a variant of green fluorescent protein (Turbo-GFP, Sigma-Aldrich, St. Louis, MO) were packaged in HEK-293T cells (17). The viral concentrations in the culture medium were titrated by PCR after cotransfection of HTCE cells with pCMV-VSV-G, psPAX2, and pGIPZshRNA-CTCF … cylindrical bore
CTCF Expression is Essential for Somatic Cell Viability and …
WebPara el tratamiento de la Enfermedad de Chagas existen las drogas Nifurtimox y Benznidazol (Bz) ambas con efectos adversos. Es necesario orientar la búsqueda de drogas tripanocidas identifi-cando un blanco molecular esencial en el Trypanosoma cruzi (T. cruzi) y ausente en el huésped. WebMay 21, 2024 · As shown in Figs 5C and S11, CTCF and control shRNA expressing cells showed very similar profiles of tax mRNA and RFP production. Taken together, these results suggest that loss of CTCF binding at the vCTCF-BS leads to increased viral gene expression, and that this effect is not due to loss of CTCF binding elsewhere in the host … WebNov 30, 2024 · CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. ... cylindrical boss